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Concentrations of TNF-α, IL-6, and IL-1 β were quantified by ELISA in (A) plasma and (B) kidney tissue homogenates from BF and NEC pups. Data are presented as mean ± SEM. Statistical analysis was performed using an unpaired t-test with Welch’s correction, with p values indicated on the graphs (* p < 0.05, ** p < 0.01).

Journal: bioRxiv

Article Title: Photoacoustic imaging assessment of acute kidney injury associated with experimental necrotizing enterocolitis

doi: 10.1101/2025.09.30.679399

Figure Lengend Snippet: Concentrations of TNF-α, IL-6, and IL-1 β were quantified by ELISA in (A) plasma and (B) kidney tissue homogenates from BF and NEC pups. Data are presented as mean ± SEM. Statistical analysis was performed using an unpaired t-test with Welch’s correction, with p values indicated on the graphs (* p < 0.05, ** p < 0.01).

Article Snippet: Following the manufacturer’s instructions, plasma samples were analyzed for inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) using enzyme-linked immunosorbent assay (ELISA) kits (CSB-E11987r, CSB-E04640r CUSABIO, Houston, USA, and RLB00-1 Quantikine, R&D Systems, MN, US).

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics